一、基本情况
梅萌，博士，讲师
邮箱：20220038@hubu.edu.cn
研究领域：酵母内质网滞留机制研究，蛋白酶和抗体折叠、表达以及蛋白酶分子改造。
二、教育背景
2014.09-2019.06，威廉williamhill官网，理学博士
2010.09-2014.06，黄冈师范学院化工学院，理学学士
三、工作经历
2022.06 至今 威廉williamhill官网，讲师
2019.06-2022.06 威廉williamhill官网，博士后
四、主持科研项目
国家自然科学基金青年项目：酵母细胞非HDEL型内质网滞留信号肽介导的滞留机制探索研究，24万元，2021.01–2023.12，主持
五、代表性论文（#第一作者; *通讯作者）
1. Mei M., Fan, X., Zhou, Y., Zhang F., Zhang G, Yi L*, “A combinatorial strategy for HRV 3C protease engineering to achieve the N-terminal free cleavage”. Int. J. Biol. Macromol., 265 (2024) 131066.
2. Mei, M., Lu M, Li S, Ren X, Xing B, Hu Y, Wu Y, Chen H, Wang L, Yi L, Ming K, Wei Z,“Development of nanobodies specific for clumping factors A of Staphylococcus aureus by yeast surface display”, Int. J. Biol. Macromol., 2024, 259(Pt 2): 129208.
3. Mei, M., Li, J., Wang, S., Lee, B., Iverson B. L., Zhang, G., Ge, X.,* and Yi, L.* “Prompting Fab Yeast Surface Display Efficiency by Yeast ER Retention and Molecular Chaperon Co-expression”, Front. Bioeng. Biotechnol., 2019, 26;7:362.
4. Mei, M., Zhai, C., Li, X. Z., Zhou, Y., Peng, W. F., Ma, L., Wang, Q. H., Iverson B. L., Zhang, G. M. *, and Yi, L.* “Characterization of Aromatic Residue-controlled Protein Retention in the Endoplasmic Reticulum of Saccharomyces cerevisiae”, J. Biol. Chem., 2017, 292(50):20707-20719.
5. Mei, M., Zhou, Y., Peng W. F., Yu, C., Ma, L. X., Zhang, G. M.*, and Yi, L.* “Application of Modified Yeast Surface Display Technologies for Non-Antibody Protein Engineering”, Microbiol. Res., 2017, 196:118-128.
6. He, H., Wu, S., Mei, M., Ning, J., Li, C., Ma, L., Zhang, G., and Yi, L.* A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris, Front. Bioeng. Biotechnol., 2020, 8, 118.
7. Fan, X., Li, X., Zhou, Y., Mei, M., Peng, W., Jiang, Z., Yang, S., Iverson B. L., Zhang, G.,* and Yi, L.* " Quantitative Analysis of the Substrate Specificity of Human Rhinovirus 3C Protease and Exploration of Its Substrate Recognition Mechanisms", ACS Chem. Biol., 2019, 15(1):63-73.
8. He, H., Zhai, C., Mei, M., Rao, Y., Wang, F., Ma, L., Zhang, G., and Yi, L.* "Functional Expression of Porcine Interferon-α Using a Combinational Strategy in Pichia pastoris GS115", Enzyme Microb. Technol., 2019, 122:55-63.
9. Zhang, Z., Tang, R., Bian, L., Mei, M., Li, C., Ma, X., Yi, L.,* and Ma, L.* "Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli ", PLoS One, 2016, 11(8):e0160367.
10. Zhang, L., Mei, M., Yu, C., Shen, W., Ma, L., He, J., and Yi, L.* "The Functions of Effector Proteins in Yersinia Virulence", Pol. J. Microbiol., 2016, 65(1):5-12.
11. 蔡浩宜，赵新露，梅萌，周瑜，张琳玲，沈文文，马立新，何结望，易犁.* " Dithiolopyrrolones 的合成, 广谱生物学活性及其应用", 中国抗生素杂志, 41 (6), 401-410.
六、授权专利
1. 一种滞留效应增强的内质网滞留信号肽突变体；易犁，梅萌，张桂敏，马立新，马延和；专利授权号：ZL201510658688.7。
2. 一种split-TEV-Fast系统的构建方法及其在检测蛋白相互作用中的应用；易犁，王婷， 张桂敏，梅萌，王钦宏；专利授权号：ZL201711193064.8。
3. 一种利用内质网滞留信号肽提高酵母细胞表面展示Fab片段抗原结合能力的重组载体；易犁，梅萌，李俊红，汪声晨，张桂敏；专利授权号：ZL201910523234.7。
4. 一种利用分子伴侣提高酵母细胞表面展示功能性Infliximab Fab片段的方法；易犁，梅萌，李俊红，汪声晨，张桂敏；专利授权号：ZL201910523199.9。
